Additional file 5.
Supplementary Figure S5. Analysis of septin rings in yeast strains expressing various Ulp1 constructs. Strains expressing Ulp1(3)(C580S), full-length Ulp1(WT) or Ulp1(3) were fixed and attached to glass slides. The septin Cdc11 was detected using an anti-Cdc11 antibody. Bud-neck localized Cdc11, 4', 6-diamidino-2-phenylindole-stained nuclear DNA and the overlaid images with pseudocolored red septins and blue DNA are shown. Note that all strains show bud-neck, localized, well-defined septin rings.
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Elmore et al. BMC Biology 2011 9:74 doi:10.1186/1741-7007-9-74