Additional file 1.

Supplementary Figure S1. (A) Identification of important features required for Ulp1 targeting and small ubiquitin-like modifier (SUMO) binding. The yeast Ulp1 catalytic domain was BLASTed against all nonredundant protein sequences in the National Center for Biotechnology Information database using psi-blast http://blast.ncbi.nlm.nih.gov/Blast.cgi webcite. After seven iterations, the top 100 query sequences (only 11 are shown) were aligned, which included a variety of animal, plant and fungal species. Residues that constitute the SUMO-binding surface (SBS) are shown in red. Also indicated are the salt bridge forming D451 and one of the residues mutated in the ulp1ts allele, N450. Conservation: Conservation of amino acid properties. Quality: Alignment quality based on Blosum 62 scores. High values suggest no or conservative mutations. Consensus: Percentage identity. All values were calculated using Jalview http://www.jalview.org/ webcite[59]. (B) Consensus SBS based on the alignment of 250 sequences from 81 species. The height of the letters corresponds to the frequency of the amino acid in the alignment. The width is based on the proportion of sequences that contain a character (many gaps lead to narrow letters). Also indicated are the salt bridge forming D451 and one of the residues mutated in the ulp1ts allele, N450 (WebLogo 3; http://weblogo.threeplusone.com/ webcite) [60].

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Elmore et al. BMC Biology 2011 9:74   doi:10.1186/1741-7007-9-74