Kap121-independent small ubiquitin-like modifier (SUMO)-targeting information resides in region 3 of Ulp1. kap121ts cells were transformed with plasmids expressing GFP-tagged wild-type (WT) Ulp1, Ulp1(C580S) and Ulp1(3)(C580S) under the control of the Ulp1 promoter (YOK 1487, YOK 1488 and YOK 1944). Shown are representative images indicating the localization of GFP-tagged Ulp1 constructs in large-budded cells at 30°C and 37°C, the nonpermissive temperature for kap121-ts. Arrowheads indicate the position of septin ring-localized Ulp1 constructs.
Elmore et al. BMC Biology 2011 9:74 doi:10.1186/1741-7007-9-74