Figure 5.

MG132-induced autophagy in Cas-deficient cells. (A) Cas-/- (EV) and Cas-FL MEFs were treated with 1 μM MG132 or Bortezomib for 16 hours, and LC3-II expression was examined by immunoblot. Relative intensity of LC3-II was calculated by normalizing the untreated Cas-/- (EV) control to a relative intensity of 1.0, and then dividing subsequent LC3-II band intensity by actin band intensity in the same treatment group using densitometry. (B) 293T cells transiently transfected with Cas shRNAs were treated with 1 μM MG132, and LC3-II expression was examined by immunoblot as in A. (C) LC-3 immunostaining in MG132-treated Cas-/- (EV) and Cas-FL MEFs. (D) Cas-/- (EV) and Cas-FL MEFs were treated with 1 μM MG132 in the presence or absence of 10 nM Bafilomycin A1 (BAF), and LC3-II expression was examined as in A.

Zhao and Vuori BMC Biology 2011 9:73   doi:10.1186/1741-7007-9-73
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