Additional file 6.

Identification of a putative iodoacetamide transporter by the robot-assisted experiment. (a) Control plate with homozygous deletion mutant strains spotted in quadruplicates (by a Singer RoToR© HAD robot) onto F1 medium agar plate containing 1% DMSO. (b) Identity of deletion mutants spotted onto control and drug plates. (c) Homozygous deletion mutant strains spotted in quadruplicates (by a Singer RoToR© HAD robot) onto F1 medium agar plate containing 20 μM iodoacetamide. (d) Quantification of the relative growth between drug and control plates with iodoacetamide resistant strains highlighted in green.

Format: PPT Size: 597KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Lanthaler et al. BMC Biology 2011 9:70   doi:10.1186/1741-7007-9-70