Additional file 24.
F1 minimal medium. Components I, II and III can be made up together at 5× final concentration and autoclaved. Component III can be made up at 5× final concentration and autoclaved. Component IV (vitamin solution) is filter-sterilized and kept at -20°C; aliquots are added to fresh 1× solution. Component V is made up as 40% w/v stock solution and autoclaved.
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Lanthaler et al. BMC Biology 2011 9:70 doi:10.1186/1741-7007-9-70