Additional file 18.
Recruitment of Irga6 mutants to the T. gondii PVM. Irga6-deficient MEFs were stimulated with IFNγ and transiently transfected with Irga6-cTag1 WT and mutant constructs. The cells were infected with avirulent T. gondii strain ME49. Intracellular parasites were detected with anti-GRA7 monoclonal antibody (red) and ectopically expressed Irga6-cTag1 with anti-cTag1 antiserum (green). Nuclei were stained with DAPI (blue). Irga6-cTag1 coated (arrowhead) and non-coated (arrow) parasites are indicated. Weakly coated parasites, counted as Irga6-cTag1 positive (Figure 10b), are marked with open arrowheads. Scale bar, 10 μm.
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Pawlowski et al. BMC Biology 2011 9:7 doi:10.1186/1741-7007-9-7