Additional file 16.
The 3'OH of the GTP ribose is essential for oligomerisation; the 2'OH is not required for cooperative hydrolysis. (a) Oligomerisation of 80 μM WT Irga6 protein was monitored in the presence of 10 mM GTP, 2'dGTP, 3'dGTP or 2'3'ddGTP by DLS at 37°C. (b) Hydrolysis of 10 mM GTP or 2'dGTP was measured after 30 min. in the presence of various concentrations of WT Irga6 protein at 37°C. Samples were assayed by HPLC.
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Pawlowski et al. BMC Biology 2011 9:7 doi:10.1186/1741-7007-9-7