The catalytic interface is involved in Irga6-Irgb6 and Irga6-Irgm3 interactions. Pull-down of Irgb6 (a) and Irgm3 (b) with recombinant GST-tagged Irga6 protein from IFNγ-stimulated gs3T3 fibroblasts lysate in the presence or absence of guanine nucleotides (0.5 mM GDP, GTPγS or mant-GDP). GST-Irga6 protein was visualised by Ponceau S staining upon blotting (top rows). Irgb6 and Irgm3 were detected with anti-Irgb6 and anti-Irgm3 monoclonal antibodies (bottom rows). A shorter exposure of the lysate input is shown. Dotted lines indicate positions where irrelevant lanes were removed from the image.
Pawlowski et al. BMC Biology 2011 9:7 doi:10.1186/1741-7007-9-7