The nucleotide ribose is part of the catalytic interface. (a) Mant-GTP; the mant-group is attached via the 2' and the 3'oxygen to the GTP ribose respectively. The attachment places are indicated by dotted arrows. (b) Oligomerisation of 80 μM WT Irga6 protein was monitored by light scattering in the presence of 10 mM GTP or mant-GTP at 37°C.
Pawlowski et al. BMC Biology 2011 9:7 doi:10.1186/1741-7007-9-7