The catalytic interface is required for efficient targeting of Irga6 to the T. gondii PVM. Irga6-deficient MEFs were stimulated with IFNγ and transiently transfected with an Irga6-cTag1 construct. The cells were infected with the avirulent T. gondii strain ME49. Intracellular parasites were detected with anti-GRA7 monoclonal antibody and ectopically expressed Irga6 with anti-cTag1 antiserum. (a) Representative images of the WT and a mutant of the catalytic interface are shown. Irga6 coated (arrowhead) and non-coated (arrow) parasites are indicated. Scale bar, 10 μm. (b) Irga6 positive PVMs were counted among the total amount of intracellular parasites. The mean values of two independent experiments are shown. The error bars indicate the standard deviation between individual experiments.
Pawlowski et al. BMC Biology 2011 9:7 doi:10.1186/1741-7007-9-7