BbtCaspase-1/2 translocated from nucleus to cytoplasm dependent on bbtASCs. (A) Comparison of the domains of hsASC with those of the bbtASC-S and the bbtASC-L. (B) BbtASC-S and bbtASC-L both led to bbtCaspase-1/2 translocation from nucleus to cytoplasm and co-localization. (C) Co-immunoprecipitation experiments performed in HeLa cells confirmed that bbtCaspase-1/2 interacted with bbtFADD2 and bbtASCs. The prodomain of bbtCaspase-1/2 (C2-2) interacted with DD of bbtFADD2 and DED of bbtASCs directly, but prodomains of bbtCaspase-8 (C8-1) and bbtCaspase3-like (C3-1) did not. WB: antibody used in Western blotting analysis. Data show a representative result from at least three separate experiments.
Xu et al. BMC Biology 2011 9:60 doi:10.1186/1741-7007-9-60