Notch reporter's response to ectopic Notch signalling. (A-C, G) In vivo time course analysis of fluorescence intensity in embryos co-electroporated with pHes5-VNP, pCAG-CherryNLS and pCAG-NICD (A-C), or pHes5-VNP and pCAG-CherryNLS (G), shows that VNP expression is higher in the presence of NICD. (D-F, H-J) Analysis of transverse sections of embryos electroporated with pHes5-VNP, pCAG-CherryNLS and pCAG-NICD (D-F), or pHes5-VNP and pCAG-CherryNLS (H-J) and harvested 24 hours post transfection, reveals that in the presence of NICD virtually all electroporated cells express VNP. (K) Chart showing the percentage of electroporated cells (CherryNLS+) expressing VNP under the Hes5-1 promoter in the absence (31.7%) or presence (~100%) of NICD.
Vilas-Boas et al. BMC Biology 2011 9:58 doi:10.1186/1741-7007-9-58