Table 2 

Cell morphology and cytoskeleton organisation in PMMdepleted PC3 cells. 

Group 
Gene symbol 
Actin 
Microtubules 
Cell shape 
Spread area 
Multinucleate 


control 
stress fibres 4% strong patches 8% cortical actin 12% low 14% 
processes 6% 
elongated 10% rounded 4% 
larger 4% 
0% 

1 
ARC 
stress fibres 70% 
NP 
elongated 
larger 
6% 
ZRANB1 
stress fibres 90% 
NP 
elongated 
larger 
10% 

2 
C20orf142 
cortical actin 
NP 
NP 
larger 85% 
NP 
CAMSAP1 
cortical actin, spots 
NP 
NP 
larger 85% 
NP 

FMNL1 
cortical actin 90%, rough edge 
NP 
NP 
larger 
NP 

FAM40A 
cortical actin 90%, rough edge 
NP 
NP 
NP 
NP 

C21orf2 
cortical actin 55% 
NP 
NP 
NP 
NP 

3 
FMNL2 
cortical actin, spots 
processes 60% 
NP 
larger 
9% 
ZNF135 
spots 
processes 50% 
NP 
NP 
NP 

ZMYM3 
cortical actin, spots 
processes 25% 
NP 
larger 
NP 

PHIP 
cortical actin 
processes 35% 
NP 
NP 
NP 

4 
FAM40B 
cortical actin 
processes 
elongated 65% 
NP 
NP 
FNBP3 
cortical actin, stress fibres 
processes 
elongated 65% 
larger 
4% 

FMNL3 
NP 
processes 
elongated 80% 
larger 
NP 

Rac1 
stress fibres 
NP 
elongated 65% 
larger 
NP 

LIMD1 
NP 
processes 
elongated 50% 
NP 
NP 

SH3KBP1 
NP 
NP 
elongated 90% 
NP 
NP 

RhoA 
strong patches 
NP 
elongated 70% 
NP 
NP 

SH3D19 
spots 
NP 
very elongated 90% 
NP 
NP 

5 
ZMYM4 
spots 
processes 
very elongated 95% 
NP 
NP 
WAVE3 
spots 
processes 
very elongated 90% 
NP 
NP 

LARP4 
NP 
processes 
very elongated 45% 
NP 
NP 

DIAPH1 
NP 
processes 
very elongated 90% 
larger 
NP 

RhoU 
NP 
NP 
very elongated 80% 
NP 
NP 

6 
CAPZB 
strong patches 90%, cortical actin 
NP 
NP 
NP 
NP 
CFL1 
strong patches 80% 
NP 
NP 
NP 
31% 

PDZK8 
strong patches 45% 
NP 
NP 
NP 
2% 

7 
ZMYM6 
rough edge 
NP 
rounded 65% 
NP 
NP 
Cdc42 
rough edge 
processes 
rounded 70% 
NP 
NP 

WTIP 
NP 
NP 
rounded 95% 
NP 
NP 

BRWD1 
NP 
NP 
rounded 80% 
smaller 
NP 

BRWD3 
NP 
NP 
rounded 80% 
smaller 
NP 

HYPC 
low 65%, smooth 
NP 
NP 
NP 
NP 

EPB41L4A 
NP 
NP 
NP 
NP 
NP 



Phenotypes were classified three days after transfection with siRNA pools targeting each of the 26 PMMs or 8 known regulators of the actin cytoskeleton (italics). Cells were fixed and stained for Factin, microtubules, and DNA (as in Figure 1). Three to four different images from two different screens were analysed; total number of cells analysed/condition ≥ 68. The most prominent phenotype for each gene is highlighted in bold text and the percent of cells showing this phenotype is shown in brackets (see Figure 1, Supplementary. Figure 2 for examples of each phenotype). Definitions of actin phenotypes: stress fibres, multiple thick straight Factin cables/cell; cortical actin, increased Factin on regions of the plasma membrane; spots, small bright regions of Factin; strong patches, large bright regions of Factin; rough edge, free edges of cells are wavy or indented; smooth, Factin levels similar throughout cytoplasm; low, lower Factin levels than control cells. Microtubule phenotype: processes, long narrow membrane extensions containing microtubules. Definitions of cell shape: larger, spread area increased compared to control cells; elongated, cell body length ≥ 2 × width; very elongated, cell body length ≥ 3 × width; rounded, length ~ = width. NP, no phenotype. 

Bai et al. BMC Biology 2011 9:54 doi:10.1186/17417007954 