Figure 2.

Effects of PMMs on migration into scratch wounds. PC3 cells were transfected with siRNA pools for each PMM in 96-well plates. After 72 h, cells were scratch wounded and then wound closure monitored by time-lapse microscopy. (A) Examples of images from control siRNA-transfected cells. Phase contrast images (left panels). To quantify wound closure, phase-contrast images were thresholded (right panels). (B) Example graph of wound closure for seven PMMs and control. Using thresholded images, the relative wound area is determined at each time point. A.U., arbitrary units. (C) Relative velocity of cells migrating into wound, normalized to control. PMMs are shown as black bars; known regulators of actin dynamics as grey bars. Those above control siRNA (white bar) stimulated migration; those below inhibited migration. Results show the mean +/- s.d. of four to eight wound areas from two experiments; * P ≤ 0.05, ** P ≤ 0.01, ***P ≤ 0.001 compared to control, two-tailed unpaired t-test.