Figure 1.

The GRASP assay for detecting synapse formation between specific neurons. Top: the two halves of split GFP are localized to pre- and postsynaptic specializations, respectively. Only when a synapse is formed will the complementary parts of GFP come close enough to reconstitute a fluorescent molecule (left). Bottom: GRASP used as a readout for synapse formation between the PHB and AVA neurons. Fluorescent puncta are only detected when synapses are formed between PHB and AVA (left).

Ch'ng BMC Biology 2011 9:43   doi:10.1186/1741-7007-9-43
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