Figure 1.

Deconvoluted electrospray ionization time-of-flight mass spectography spectra of the different metal-metallothionein (MT) complexes recombinantly synthesized: (a) HpCdMT obtained in Cd-enriched medium; (b) HpCdMT produced in Zn-enriched medium; and (c) HpCuMT synthesized in Cu-enriched medium under low aeration conditions. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis of these preparations indicated a respective mean content of 6.2 Cd/MT in (a) and 5.8 Zn/MT in (b) for HpCdMT and of 12.2 Cu/MT for HpCuMT in (c).