Data production workflow for microarrays. Microarrays require labeling of target material, hybridization to arrays, washing, and scanning to obtain measures of gene expression. RNA converted to cDNA from the sample will hybridize to the corresponding oligonucleotide targets, so that more highly expressed genes will be reflected in more abundant material hybridized and thus greater fluorescence intensity. In modern arrays, multiple probes are designed for a single gene in order to obtain fluorescence intensities that can be used as an index of gene expression.
Malone and Oliver BMC Biology 2011 9:34 doi:10.1186/1741-7007-9-34