Additional file 4.

Figure S3. Mutations at Rpn1 residues A418, N549, F565 and G571 render unstable proteasomes Pre1-myc13 tagged proteasomes from strains carrying plasmid borne Rpn1 alleles in an RPN1 null strain, were immunoprecipitated from whole cell extracts and analyzed by immunoblotting with the indicated antibodies. As shown, proteasomes with mutations at residues A418, N549, F565 and G571 exhibit dissociation of the 19S cap with the proteasomal base during immunoprecipitation experiments.

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Gomez et al. BMC Biology 2011 9:33   doi:10.1186/1741-7007-9-33