Additional File 8.

Overview of observed fluorescence resonance energy transfer (FRET) ratio changes Histogram depicting the normalized FRET ratio (yellow fluorescent protein:monomeric Turquoise (YFP:mTq)) changes upon histamine stimulation, observed in cells expressing Gαq-mTqΔ6, Gβ1 and YFP-Gγ2 (wild-type (wt); n = 54); cells expressing Gαq-mTqΔ6, Gβ1, YFP-Gγ2 and histamine 1 receptor (H1R, n = 8); cells expressing Gαq-mTqΔ6, Gβ1, YFP-Gγ2 and the guanine nucleotide exchange factor p63RhoGEF (p63, n = 29); cells expressing Gαq-mTqΔ6, Gβ1, YFP-Gγ2 and p63RhoGEF-L475A (p63LA, n = 15). The error bars depict SE.

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Adjobo-Hermans et al. BMC Biology 2011 9:32   doi:10.1186/1741-7007-9-32