Additional File 2.
Expression of Gαq in mouse embryonic fibroblasts (MEF) and HeLa cell lines Transfected, transduced or untreated cells were treated with trypsin and lysed. Each lane was loaded with 40 μg of protein. Western blotting was performed with an antibody against Gαq/11 (diluted 1:1000) and exposed for 30 seconds. Lane 1 contains wild-type HeLa cells; lane 2 contains HeLa cells transfected with Gαq-mTqΔ6; lane 3 contains MEFq/11-/- cells; lane 4 contains MEFq/11-/- cells transduced with Gαq-mYFP and lane 5 contains wild-type MEF cells.
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Adjobo-Hermans et al. BMC Biology 2011 9:32 doi:10.1186/1741-7007-9-32