Additional File 10.

PtdIns(4,5)P2 hydrolysis upon hypotonic stimulus (A) Green fluorescent protein (GFP)-PH was used as an indicator for PtdIns(4,5)P2 levels, and was found to be localized predominantly at the plasma membrane of HeLa cells. (C) Membrane localization was quantified by means of a line profile plot. Upon applying the hypotonic stimulus (B) the PH domain translocated partially to the cytoplasm, which is also evident from (D) the line profile plot, showing decreased membrane intensity and increased cytoplasmic intensity.

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Adjobo-Hermans et al. BMC Biology 2011 9:32   doi:10.1186/1741-7007-9-32