Gel filtration chromatography of TkoGINS complexes. (A) Elution profiles of the wild type (thin line) complex and a truncation mutant (thick line) complex lacking the Gins51 C-terminal domain (Gins51ΔC: Met1-Arg130) (upper part). The elution positions of the marker proteins, thyroglobulin (670 K), immunoglobulin G (158 K), ovalbumin (44 K), and myoglobin (17 K), are shown by arrows on the top. The elution volume (ml) for each molecule is indicated on the top with the apparent molecular mass shown in parenthesis. Aliquots of each fraction were subjected to 12.5% SDS-PAGE analysis followed by Coomassie Brilliant Blue staining (lower part). (B) Gel filtration chromatography and SDS-PAGE of the isolated Gins51, Gins23 and Gins51ΔC.
Oyama et al. BMC Biology 2011 9:28 doi:10.1186/1741-7007-9-28