Figure 8.

Diagram of barcoded amplicon. The amplicon of interest, flanked by forward and reverse PCR primer sequences, was subjected to ligation of the Titanium Adapter B and Adapter A + barcode sequence, producing an oligonucleotide of the form shown. One out of 64 barcode + check-sum base sequences is represented here. Initially, the Adapter A oligonucleotide used was 6 bp shorter at the 5' end. It was extended by PCR to match the Titanium sequence shown here, as described in the text.