Female sexual attraction to intact urine or to the high molecular weight fraction of urine (≥ 3 kDa, HMW) according to stimulus age. Urine stimuli (10 μL) from male (blue bars) and female (pink bars) B6 mice were streaked onto 55 mm diameter glass microfibre filters cut in half and left to dry at room temperature for 5 min, 24 h or 7 days prior to testing. The effect of stimulus age and donor sex on response (log s + 1) to intact urine or to HMW was compared by repeated measures ANOVA with stimulus sex as a within-subjects factor and urine age as a between-subjects factor. P values on the graph show post-hoc t-tests which confirmed greater response to the male sample within each test. Log transformed data are plotted to illustrate the similarity of response between tests.
Roberts et al. BMC Biology 2010 8:75 doi:10.1186/1741-7007-8-75