The genetic organization of TOMM and lanthionine biosynthetic clusters that utilize NHase- and Nif11-related precursor peptides. Genomic regions are shown from selected organisms in which the precursor peptides are clustered with the cognate modification enzymes. In most cases, a transport system is also visible in the local region. The TOMM precursors represented by Burkholeria cenocepacia (dark gray ORFs, NHLP-Burk) are accompanied by a large, Ser/Thr kinase. Highly similar clusters have been identified in Acidovorax avenae and Delftia acidovorans. In several species, precursors shown in black (NHLP) and light grey [Nif11-derived peptide (N11P)] may cluster with each other as well as with other modification and transporter genes. Note: only those precursors closest to the cyclodehydratase-docking scaffold protein or LanM-like lanthionine synthase are shown. For instance, Pelotomaculum thermopropionicum has 12 NHLPs (only two are shown) and seven N11P family precursors, while Cyanothece sp. PCC 7425 and P. marinus have 18 and 29 predicted N11P precursors, respectively (eight and seven are shown). Transport proteins, including those homologous to HlyD (type I secretion, purple) and ABC transporters (red), correspond to the transport genes detected by PPP.
Haft et al. BMC Biology 2010 8:70 doi:10.1186/1741-7007-8-70