FISH mapping of BAC clones. A) Single color FISH mapping of TGAC-157B03 reveals extensive cross-hybridization across chromosomes. Similar results were observed for other Class II clones presumably as a result of their high repeat content. B) Lack of cohybridization between Clones TGAC-102M22 and a known chromosome 22 BAC indicates that TGAC-102M22 is not on chromosome 22 as indicated by the genome assembly. C) Dual color FISH of TGAC-86I22 (red) and TGAC-167E04 (green) indicating cohybridization of these clones, a result also supported by sequence analysis. These clones were assembled together, and contain g-filamin, TNXB, TAP1 and TAP2 genes. D) Clones TGAC-102M22 (red) (contains MHC Class I, FLOT, TUBB, KIFC and DAXX) and TGAC-86I22 map to different chromosomes. Key components of the classical MHC therefore map to different chromosomes in the zebra finch genome.
Balakrishnan et al. BMC Biology 2010 8:29 doi:10.1186/1741-7007-8-29