Table 4

Identification of peptides in the four major song nuclei.

Mass

Putative Identity

Prohormone

Detected


Area X

LMAN

HVC

RA


877.4

YGGFMSSE

POMC


YGGFMRF

PENK


1375.9

RPRPQQFFGLMamide

TAC1

-


WNKMDELAKQL

CHGA


1415.8

SEYRGHLPAEKE

SCG1

-

-

-


VGGASGGLGDDAEPLT

ADCYAP1


1448.8

VGRPEWWLDYQ

PENK

-


1477.0

WNKMDELAKQLT

CHGA

-

-

-


1529.0

GKTNRYDVERQY

SCG1

-

-

-


1558.9

HSDAVFTDNYSRF

VIP

-

-

-


1591.2

p-QLHVNKARRPYIL

NTS


1608.1

MPLGHYEDSSRDSPF

SCG2


1740.2

NPGKTNRYDVERQY

CHGB

-

-

-


1773.1

SVNPYLQGQRLDNVVA

SCG5

-

-


2127.5

DSAGEQLPEGREEGRPALSE

CHGB

-

-


4966.1

Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES

TMSB


The mass listed is the observed mass [M+H]+ from the MALDI TOF mass spectrometry of the HVC samples. The peptide identities of 13 peaks in the mass spectrum of the HVC homogenate were assigned based on mass matches to the previously identified peptides. Three peaks (877.4, 1375.9, and 1415.8 m/z) were assigned to two putative peptides each. A check mark (√) indicates the song nucleus where the masses were observed. A dash (-) indicates it was not detected.

Xie et al. BMC Biology 2010 8:28   doi:10.1186/1741-7007-8-28

Open Data