Table 3

Microarray and in situ hybridization (ISH) results for prohormone expressed sequence tags (ESTs).

Prohormone/gene name

Gene symbol

Accession No.

AASS P-value

SASS P-value

AASA P-value

ISH P-value


Adenylate cyclase activating polypeptide 1 (pituitary)

ADCYAP1

CK303710

0.46

0.64

0.78

-

Adenylate cyclase activating polypeptide 1 (pituitary)

ADCYAP1

CK303710

0.91

0.56

0.64

-

Adrenomedullin

ADM

DV955971

<0.01

0.67

<0.01

0.58

C-RF amide peptide

CRF

DV949637

0.69

0.43

0.69

-

Cholecystokinin

CCK

CK302967

<0.01

0.72

<0.01

0.05

Chromogranin A (parathyroid secretory protein 1)

CHGA

DV950480

0.07

0.06

0.96

-

Chromogranin B (secretogranin 1)

CHGB

CK308123

0.14

0.22

0.79

-

Chromogranin B (secretogranin 1)

CHGB

CK308816

0.01

0.14

0.21

-

Chromosome 2 open reading frame 40

C2orf40

CK314083

0.10

0.19

0.72

-

Corticotropin-releasing hormone

CRHR1

CK309161

0.05

0.02

0.61

-

Endothelin 3

EDN3

DV946954

0.84

0.47

0.61

-

Galanin prepropeptide

GAL

DV957373

0.23

0.24

0.03

-

Gastrin-releasing peptide

GRP

CK312472

0.02

0.23

0.20

-

Glycoprotein hormones, alpha polypeptide

CGA

CK310677

0.77

0.94

0.83

-

Growth hormone 1 (Chr27)

GH1

CK301315

0.04

0.20

<0.01

0.04

Growth hormone 1 (duplicate on Chr1)

GH1A

DV950694

0.42

0.11

0.40

-

Hypocretin (orexin) neuropeptide precursor

HCRT

DV955493

0.06

0.42

0.26

-

Insulin-like growth factor 1 (somatomedin C)

IGF1

DV948990

<0.01

0.14

0.01

0.11

Islet amyloid polypeptide

IAPP

CK234383

0.57

0.57

0.27

-

Neuromedin B

NMB

DV950473

0.82

0.41

0.30

-

Neuromedin B

NMB

DV959623

0.71

0.03

0.07

-

Neuropeptide W

NPW

DV945212

0.61

0.19

0.41

-

Neuropeptide W

NPW

DV945212

0.34

0.22

0.78

-

Neuropeptide Y

NPY

CK310313

<0.01

0.28

<0.01

0.54

Neurotensin

NTS

CK302282

0.06

0.11

<0.01

0.06

Neurotensin

NTS

CK305091

0.01

0.11

<0.01

-

Platelet-derived growth factor alpha polypeptide

PDGFA

DV959989

0.06

0.88

0.04

-

Prepronociceptin

PNOC

CK234392

0.06

0.03

<0.01

-

Proenkephalin

PENK

CK301334

0.08

0.68

0.04

-

Proenkephalin

PENK

CK312129

0.16

0.94

0.18

-

Secretogranin II (chromogranin C)

SCG2

CK313614

0.86

0.27

0.35

-

Secretogranin II (chromogranin C)

SCG2

DV945506

0.50

0.14

0.39

-

Secretogranin V (7B2 protein)

SCG5

CK313144

0.21

0.04

0.36

-

Somatostatin

SST

CK234915

<0.01

0.65

<0.01

-

Somatostatin 2

SST2

CK314968

0.71

0.11

0.05

-

Tachykinin, precursor 1

TAC1

CK301285

0.01

0.73

0.03

-

Tachykinin, precursor 1

TAC1

DV958953

0.18

0.20

0.02

-

Thyrotropin-releasing hormone

TRH

CK302320

0.18

0.20

0.95

-

Urocortin

UCN

DV950835

0.336

0.285

0.908

-

Urotensin 2 domain containing

UTS2D

DV945629

0.11

0.10

0.99

-

Vasoactive intestinal peptide

VIP

DV958568

0.07

0.07

0.94

-


Forty-one unique ESTs that represent 32 prohormone genes were identified on the zebra finch brain 20K SoNG microarray. Some ESTs were spotted twice on the array, and in some cases more than one EST are from a single prohormone; all ESTs are included. Listed are the raw P-values from an experiment that compared expression profiles in the adult male auditory forebrain after birds were exposed to three auditory stimuli: (AA, indicating the bird was habituated to song A on one day then tested for its response to the song on the next day), novel song (SA, indicating the bird heard silence on the first day and then song A for the first time on the day of testing) or silence (SS, indicating the bird heard silence both days) [44]; a false discovery rate (FDR) significant cutoff of FDR < 0.05 was used to select genes for ISH analysis. Comparisons are: birds that heard either familiar song versus those that heard silence (AASS), birds that heard novel song versus those that heard silence (SASS), or birds that heard familiar song versus those that heard novel song (AASA). Six ESTs that were reported to show a significant change in the familiar group [44] were used as riboprobes for ISH analysis. Of these six, three ESTs showed a significant change in the number of cells with modulated expression levels related to song experience. Significant P-values (P < 0.01 for microarray; P < 0.05 for ISH values) are in bold. A dash (-) indicates that the EST was not investigated using ISH.

Xie et al. BMC Biology 2010 8:28   doi:10.1186/1741-7007-8-28

Open Data