Figure 6.

Expression and creatine kinase (CK)-binding specificities of endogenous human ankyrin repeat and suppressor of cytokine signalling (SOCS) box protein 9 (ASB9). (a) Expression of endogenous ASB9 and ASB9ΔSOCS. Cell lysates of human embryonic kidney 293 (HEK293) cells and Huh-7 cells were used to show endogenous expression of ASB9 and ASB9ΔSOCS. Endogenous expression of CKB, mitochondrial CK (MtCK), elongin B was also confirmed by immunoblotting. (b) CK-binding activity of endogenous ASB9. The immunoprecipitates obtained with the anti-ASB9 antibody were analysed by immunoblotting (IB) with anti-ASB9, anti-CKB, anti-uMtCK, and anti-elongin B antibodies.