Table 1

Protein sequences of pectinolytic enzymes in ant fecal fluid and corresponding molecular weights in daltons

Protein

Mass spectrometry dataa

Sequence data

Bandb

MW (Da)


Arabinofuranosidase

FPGGNNJENTVDQR

FPGGNNLENTVDQR

4

69139

GD[YQ/QY]JPSTJPSSTGTVFWSVVR

GDQYIPSTLPSSTGTVFWSVVR

NDJASAJAEVG[SP/PS]FWR

NDIASALAEVGPSFWR

PEDFAANTYTFR

NEDFFAANTYTFR

STAJPNAJHVVJPTGR

STALPNALHVVIPTGR

VESAAGEAAFMTGJER

VESAAGEAAFMTGLER

YFE[W/GE]YAAJSTNNP/PNDJFG

YFEGEYAAISTNPNDIFG

Endogalactanase

DJDGJNTQJFTYTR

DLDGLNTQIFTYTR

7

37416

GAVTPFEEJJHNHGA[J/N]

GAVTPFEELLHNHGAN

GWFSSJANJESSGR

GADFSSLANLESSGR

Pectin esterase

GQAYFGGNTJ[QR/GVK]

GQAYFGGNTLGVK

6 + 7 + 8 + 9 + 10 + 18 + 19 + 22

37571

GAGWVTASGR

GAGWVTASGR

GYJEGATDFJFGQR

GYIEGATDFIFGQR

NNQATJQFGJDAGQAGSDDASGTJR

NNQATIQFGLDAGQAGSDDASGTLR

NTFGVGSQAJAJSQYGDR

NTFGVGSQAIALSQYGDR

QAYFGGNTJGVK

QAYFGGNTIGVK

Pectate lyase

JVJJSGNJSGDAVVR

IVLLSGNLSGDAVVR

7 + 8

VJNENNVJJR

VLNENNVIIR

Polygalacturonase

VAVN(1710)...(1206)TGTWNWSNJK

VAVNCGVGSCTGTWNWSNLK

6

37055

Rhamnogalacturonan acetylesterase-1

PDNJWVNGEJGAGPR

PDNIWVNGEIGAGPR

10 + 16 + 17 + 18 + 19

26946

FVGYAQTAASR

FVGYAQTAASR

VNDAJAGR

VNDAIAGR

Rhamnogalacturonan acetylesterase-2

FVTYAQSJGJR

FVTYAQSLGLR

18

26467


aThe letter J symbolizes the amino acids leucin and isoleucin, which have the same molecular mass and therefore are indiscernible by mass spectrometry. bThese numbers refer to the bands excised from the SDS-PAGE gel (Figure 1) in which the protein in question was found.

Schi√łtt et al. BMC Biology 2010 8:156   doi:10.1186/1741-7007-8-156

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