Additional file 4.

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Movie 4 - Coronin dynamics relative to actin polymerization and depolymerization. The cell taking up a budded yeast particle expresses GFP-coronin (green) and mRFP-LimEΔ (red). The movie is shown twice: first with the fluorescent labels superimposed on the greyscale brightfield images, subsequently without the brightfield images. During cup extension, coronin localizes to the boundary of the actin layer bordering the cytoplasm. After the completion of uptake, coronin persists during the period of actin disassembly. Images from this sequence are presented in Figure 3a. Frame to frame interval is 2 seconds. All Additional files are movies showing phagocytes of Dictyostelium discoideum that interact with living yeast particles. Bars in the movies indicate 10 μm.

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Clarke et al. BMC Biology 2010 8:154   doi:10.1186/1741-7007-8-154