Figure 7.

Cortexillin depletion in phagocytic cups. The external portions of the budded yeast, which are not visible in the fluorescence images, are denoted by circles. The cells are expressing mRFP-LimEΔ for actin or mRFP-ArpC1 for the Arp2/3 complex (upper panels) together with GFP-cortexillin I (lower panels). (a) Three examples of uptake of budded yeast: (left panel) an early stage of uptake, (middle) an intermediate stage, and (right) completed uptake. At all stages, cortexillin I is uniformly depleted from the phagosome membrane, whereas actin and the Arp2/3 complex are enriched around the neck of the particle. (b) Sequence showing severing of a budded yeast with strong accumulation of actin around the neck of the particle. Cortexillin I is depleted from the entire cup at all stages of severing. Numbers indicate time in seconds. Bar = 10 μm for all images.

Clarke et al. BMC Biology 2010 8:154   doi:10.1186/1741-7007-8-154
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