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Open Access Research article

Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells

Elizabeth Rico-Bautista1*, Chih-Cheng Yang1, Lifang Lu12, Gregory P Roth3 and Dieter A Wolf1*

Author Affiliations

1 Signal Transduction Program, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA

2 GE Healthcare, Life Sciences, Shanghai 201203, China

3 Sanford-Burnham Medical Research Institute at Lake Nona, Orlando, FL 32827, USA

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BMC Biology 2010, 8:153  doi:10.1186/1741-7007-8-153

Published: 23 December 2010

Additional files

Additional file 1:

Supplementary Table 1. The table shows 176 small molecule inhibitors of p27 depletion (SMIPs) identified in the primary screening. Compounds were classified according to their Z factors as strong (S), medium (M) or weak (W). Their position in the screening (stock plate and well), simplified molecular input line entry specification (SMILES), name and vendor are also listed. A column with known biological actions is also shown for some compounds.

Format: XLS Size: 89KB Download file

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Additional file 2:

Supplementary Table 2: The table shows the cell cycle distribution of LNCaP-S14 transfected with small interfering RNA for p27 and p21 and treated with SMIP004 (40 μM) as described in the Methods section. Cell were analysed by flow cytometry and the percentage of cells in the different phases of the cell cycle were calculated.

Format: XLS Size: 28KB Download file

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Open Data