Table 1

Detection of anti-inflammatory activity using the ChIN assaya

Compound

Mode of action

Concentration

(μM)

Significant effect

Reference


Ibuprofen

COX inhibitor

1

***

[38]

10

***

50

*

Diclofenac

COX inhibitor

1.5

***

[38,39]

3

***

SP600125

JNK inhibitor

20

*

[40]

50

**

100

**

200

***

Trans-resveratrol

COX-1 inhibitor

1

-

[41]

10

**

100

***

Mifepristone (RU486)

Progesterone and GR antagonist

1

-

[42]

10

**

100

***

Dexamethasone

Steroidal nitric oxide synthase inhibitor

10

-

[43]

100

**

1,000

***

Indomethacin

COX inhibitor

1

**

[39]

10

***

100

***

Rosiglitazone

PPAR-γ agonist

0.5

*

[44]

1

**

10

*

Aspirin

COX inhibitor

10

-

[39]

20

**

Hydrocortisone

Steroidal GR agonist

1

-

[45]

10

-

100

-

300

*

Sulindac

NS COX-1 inhibitor

1

**

[46]

10

***

50

***

100

***


aSelected drugs were added to the incubation medium 1 hour prior to addition of copper and were tested at the indicated concentrations for inhibition of leukocyte migration using BACmpx::GFP larvae in chemically induced inflammation assays (ChIn) (10 μM CuSO4 for 40 minutes). Up to four concentrations were chosen to provide an overview of drug activity in the ChIn assay. We aimed at identifying a concentration yielding significant results with P < 0.001. In some instances, this was not possible (rosiglitazone, aspirin, hydrocortisone) as higher concentrations were lethal or showed reduced significance compared to lower concentrations. All drugs were used in medium containing 1% dimethyl sulfoxide, as were control fish. Asterisks indicate significant leukocyte migration inhibition. ***P < 0.001. **0.001 <P < 0.01. *0.01 <P < 0.05. Minus sign indicates no significant difference. GR, glucocorticoid receptor; NS, nonsteroidal; COX, cyclooxygenase; JNK, c-Jun N-terminal kinase; PPAR-γ, peroxisome proliferator-activated receptor-γ.

d'Alençon et al. BMC Biology 2010 8:151   doi:10.1186/1741-7007-8-151

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