An example of very long transcribed intergenic regions identified in tumour cells. An example of a locus with high expression between annotated genes on chromosome 7 that was found in several Ewing Family of Tumours (EFT) cell lines and tissues but not in K562 or normal tissues is shown in panel A with chromosomal position along the X axis. A locus on chromosome 21 that was found to have high expression in K562 but not EFT samples or normal tissues is shown in panel B. The EFT primary No,1 and metastatic No.1 samples correspond to the CHLA-9 and CHLA-10 cell lines (see the Materials and Methods Section of the paper) and the remainder are from patient EFT samples, K562 cells, or normal tissues (liver and brain). The K4-K36 domains which harbour large intergenic non-coding RNAs, as reported by Khalil et al. , are also shown. The Y axis show the density of reads per each genomic base in 10 million non-ribosomal, non-mitochondrial reads. The chromosome (chr) of origin and strand of a transcript (+) or (-) are indicated.
Kapranov et al. BMC Biology 2010 8:149 doi:10.1186/1741-7007-8-149