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## Figure 1.
The insulin-signalling mutant . In this experiment daf-2(m41) has a distinctive metabolic profilem41 and N2 wild-type worms were initially raised at 15°C, transferred to 22.5°C at L4,
and assayed at 240 hours post-bleaching. A. PCA (above) on the binned spectra shows that daf-2(m41) are clearly separable from N2 along PC1 (Means and 95% CI are given by ellipses);
cluster analysis (below) separates the samples into two groups, one predominantly
wild-type (8/9), the other predominantly m41 (10/13). The first three PCs respectively account for 50, 11 and 7% of the variance.
B. Heatmap (middle) showing the standardized relative concentration of 34 bins out
of 179 with a substantial (>0.1 and <-0.1) loading on PC1, their loads (below) and
position on the ^{+1}H NMR spectrum (above). The spectrum is the median of five N2 samples; intensities
>5.0 ppm are scaled by a factor of 10.
Fuchs |