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Open Access Highly Accessed Research article

A metabolic signature of long life in Caenorhabditis elegans

Silke Fuchs12, Jacob G Bundy3, Sarah K Davies1, Jonathan M Viney4, Jonathan S Swire5 and Armand M Leroi1*

Author affiliations

1 Division of Biology, Silwood Park Campus, Imperial College London, SL5 7PY, UK

2 Current address: Division of Cell and Molecular Biology, South Kensington Campus, Imperial College London, London SW7 2AZ, UK

3 Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, South Kensington Campus, Imperial College London, London SW7 2AZ, UK

4 National Heart and Lung Institute, South Kensington Campus, Imperial College, London, London SW7 2AZ, UK

5 Centre for Bioinformatics, Division of Molecular Biosciences, South Kensington Campus, Imperial College London, London SW7 2AZ, UK

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Citation and License

BMC Biology 2010, 8:14  doi:10.1186/1741-7007-8-14

Published: 10 February 2010

Abstract

Background

Many Caenorhabditis elegans mutations increase longevity and much evidence suggests that they do so at least partly via changes in metabolism. However, up until now there has been no systematic investigation of how the metabolic networks of long-lived mutants differ from those of normal worms. Metabolomic technologies, that permit the analysis of many untargeted metabolites in parallel, now make this possible. Here we use one of these, 1H nuclear magnetic resonance spectroscopy, to investigate what makes long-lived worms metabolically distinctive.

Results

We examined three classes of long-lived worms: dauer larvae, adult Insulin/IGF-1 signalling (IIS)-defective mutants, and a translation-defective mutant. Surprisingly, these ostensibly different long-lived worms share a common metabolic signature, dominated by shifts in carbohydrate and amino acid metabolism. In addition the dauer larvae, uniquely, had elevated levels of modified amino acids (hydroxyproline and phosphoserine). We interrogated existing gene expression data in order to integrate functional (metabolite-level) changes with transcriptional changes at a pathway level.

Conclusions

The observed metabolic responses could be explained to a large degree by upregulation of gluconeogenesis and the glyoxylate shunt as well as changes in amino acid catabolism. These responses point to new possible mechanisms of longevity assurance in worms. The metabolic changes observed in dauer larvae can be explained by the existence of high levels of autophagy leading to recycling of cellular components.

See associated minireview: http://jbiol.com/content/9/1/7 webcite