Additional file 3.

Control for differentiation specificity of WT or ArtΔ/Δ MSCs. (a) Fixed ArtΔ/Δ and Art MSCs treated with adipocyte- and osteocyte-specific differentiation medium were stained with the fluorescent lipid binding dye LipidTOX. Cells grown in osteocyte-specific medium are not positive for LipidTOX staining, indicating the absence of adipocytes in these culture conditions. (b) Fixed ArtΔ/Δ and Art MSCs treated with adipocyte- and osteocyte-specific differentiation medium were stained with the mineralized bone-specific stain alizarin red. Cells treated with adipogenic medium do not stain with alizarin red, indicating that mineralized bone is not present in adipogenic-treated cells.

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Maas et al. BMC Biology 2010 8:132   doi:10.1186/1741-7007-8-132