Additional file 2.
The Art/Dclre1c, encoding ARTEMIS, is transcribed in mesenchymal stem cells (MSCs). (a) Schematic of reverse transcriptase polymerase chain reaction (RT-PCR) strategy to detect Art transcript in wild-type (WT) versus ArtΔ/Δ MSCs. PCR product detecting exons 1-4 (Art ex1-4) is common to both the WT and ArtΔ/Δ alleles (because the knockout allele eliminates exons 5-6. PCR product detecting exons 1-5 (Art ex1-5) is only amplified from WT cells, but not ArtΔ/Δ cells. (b) RT-PCR reactions detecting Art ex 1-4, Art ex 1-5, or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (control) transcripts as indicated. Shown are data for either WT or ArtΔ/Δ fibroblasts or MSCs (as indicated beneath). These data confirm detection of Art ex 1-4 in both WT and ArtΔ/Δ MSCs, but detection of Art 1-5 only in WT MSCs. This confirms transcriptional expression of Art in MSCs and verifies the expected knockout in MSCs from ArtΔ/Δ mice.
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Maas et al. BMC Biology 2010 8:132 doi:10.1186/1741-7007-8-132