Art deficiency accelerates mesenchymal tumor development. (a) Tumor-free survival analyses of ArtΔ/Δ Trp53Δ/+ mice (red circles) versus Trp53Δ/+ mice (blue, diamond). Plotted is the surviving fraction of those mice that developed tumors as a function of time (weeks). Significance was determined by t-testing. (b) Hematoxylin and eosin (H&E) staining of an anaplastic sarcoma (left) and a chondrosarcoma (center) found in ArtΔ/Δ/Trp53Δ/+ mice, shown at ×10 and ×40 magnification. Normal bone marrow from an Art mouse (right) is shown for comparison. Boxed areas in ×10 magnification demarcate regions shown in ×40 magnification. Lines represent scale bars (200 μm in ×10 magnification; 50 μm in ×40 magnification). (c) Spectral karyotype (SKY) analysis of ArtΔ/Δ Trp53+/Δ osteosarcoma. Shown are the 4',6'-diamidino-2-phenylindole (DAPI) stained metaphase (inverted image, top left) with superimposed chromosome contours (blue), spectral image of SKY painted metaphase spread (top, middle), and computer classified image (top, right), as well as the karyotype table showing approximate hyperdiploidy (bottom).
Maas et al. BMC Biology 2010 8:132 doi:10.1186/1741-7007-8-132