Additional file 8.

Additional Figure 5. KD of a subset of Klf5-target genes. (a) ESCs were stably transfected with shRNA plasmids for selected Klf5-target genes or with control shRNA (shGFP) and KD was verified by qPCR. The results are represented as fold changes relative to shGFP-transfected cells. SD of triplicates is reported. (b) Percentage of undifferentiated (blue) and differentiated (red) colonies observed by AP staining upon KD of eight Klf5-target genes with a second independent shRNA. *P < 0.01. (c) Expression levels of Oct3/4 and Nanog upon KD of eight Klf5-target genes with a second independent shRNA. The data are represented as fold changes relative to shGFP-transfected cells.

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Parisi et al. BMC Biology 2010 8:128   doi:10.1186/1741-7007-8-128