Table 3

Transgenic lines used for the analysis of tectum structure or function in zebrafish

Short name

Full name

Description

References


Pou4f3:mGFP (Brn3c:mGFP), Pou4f3:Gal4 (Brn3c:Gal4)

Tg(pou4f3:gap43-gfp)s356t, Tg(pou4f3:gap43-gfp)s273t, Tg(pou4f3:gal4-vp16)s311t

Labels a subset (40%) of RGCs; projection into SO, SFGSD and SFGSF

[10,12,22,95]

BGUG

Tg(pou4f3:gal4-vp16, UAS:gap43-gfp)s314t, Tg(pou4f3:gal4-vp16, UAS:gap43-gfp)s318t

Labels a random subset of Pou4f3-positive RGCs with membrane-bound GFP; also drives GFP expression in random cells within any Gal4 pattern ('genetic Golgi')

[12,20,21]

Ath5:GFP

(Atoh7:GFP), Ath5:mGFP, Ath5:mRFP, Ath5:GCaMP1.6, Ath5:Gal4

Tg(atoh7:gfp), Tg(Atoh7:gap43-GFP)cu1, Tg(Atoh7:gap43-RFP)cu2, Tg(atoh7:gcamp1.6), Tg(atoh7:gal4-vp16)

Labels 100% of RGCs and some retinal interneurons

[42,96-98]

Isl2b:GFP, Isl2b:mCherry-CAAX, Isl2b:mGFP

Tg(-17.6isl2b:GFP)zc7, Tg(-17.6isl2b:mCherry-HsHRAS)zc23, Tg(-17.6isl2b:gap43-GFP)zc20,

Labels all or the vast majority of RGCs

[99]

Pou4f1: GFP (Brn3a:GFP), Pou4f1:Gal4 (Brn3a:Gal4)

Tg(pou4f1-hsp70l:gfp)rw0110b

Labels RGCs. Also labels many PVNs, including glutamatergic PVPNs with ipsilateral axons to the hindbrain and GABAergic neurons with tectotectal axons

[27]

Gal4s1013t

Et(-1.5hsp70l:gal4-vp16)s1013t

Drives expression in all neurons and glia of the tectum

[20]

Gal4s1038t

Et(fos:gal4-vp16)s1038t

Drives expression in PVPNs of the posterior tectum

[20]

Gal4s1156t

Et(-1.5hsp70l:gal4-vp16)s1156t

Drives expression in very few tectal neurons, including most SINs

[54]

Gal4s1101t

Et(e1b:gal4-vp16)s1101t

Drives expression in almost all neurons of the CNS; 'pan-neural'

[55,59,60]


CNS, central nervous system.

Nevin et al. BMC Biology 2010 8:126   doi:10.1186/1741-7007-8-126

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