Additional file 2.

Effects of period (per) and cycle (cyc) RNA interference on the cuticle deposition rhythm in Riptortus pedestris. After injection of per, cyc, β-lactamase (bla) double-stranded RNA (dsRNA) or saline on the day of adult emergence, cuticle layers were observed between crossed polarizers under a light microscope. The ordinate shows the percentage of individuals with alternating double layers in the endocuticle. The experimental schedules are shown as horizontal hatched bars (short-day conditions) or open bars (long-day conditions). Arrowheads show the day of adult emergence and arrows show the day of the observation. Insects were transferred from long-day to short-day conditions (A), maintained continuously under long-day conditions (B), or transferred from short-day to long-day conditions (C). The bars with the same letters are not significantly different (Tukey-type multiple comparisons for proportions, P > 0.05).

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Ikeno et al. BMC Biology 2010 8:116   doi:10.1186/1741-7007-8-116