Additional file 1.

Gene silencing by double-stranded RNA (dsRNA) injection in Riptortus pedestris. (A) Northern blotting for period (per; left) and cycle (cyc; right) was performed using total RNA from the heads of nine females at Zeitgeber time (ZT) 8 on day 5. Ribosomal RNAs (rRNAs) are shown as references. (B) Relative transcript levels (the intensity ratios to β-tubulin) of per (left) and cyc (right) at ZT6 and 18 on day 5 quantified by northern blotting using total RNA from the whole bodies. The standard curve methodology was adopted. The levels of per in females injected with per dsRNA were not significantly different from those in females injected with β-lactamase (bla) dsRNA at both ZT6 and 18 (t- test, P > 0.05). The level of cyc in females injected with cyc dsRNA was significantly lower than that in females injected with bla dsRNA at ZT6 (t-test, P < 0.05), but not at ZT18 (t-test, P > 0.05). The highest value in each experiment was set at 1.0. Each plot represents a total RNA extracted from the whole body of a single female (N = 3) for each ZT and treatment.

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Ikeno et al. BMC Biology 2010 8:116   doi:10.1186/1741-7007-8-116