Table 1

Nystatin P1 biosynthetic genes.

Contig ID

Pseudonocardia sp. P1 protein

Proposed function*

P. autotrophica ortholog

Identity (%)


PP100949

NypF

Phosphopantetheinyl transferase

NppF

89

PP100949

NypY

Glycosyltransferase

None†

--

PP100949

NypZ

Metallophosphoesterase

None‡

95

PP100398

NypH

ABC transporter

NppH

88

PP100398

NypDIII

dGDP-mannose-4,6-dehydratase

NppDIII

96

PP100400

NypL

P450 monooxygenase

NppL

84

PP100400

NypN

P450 monooxygenase

NppN

94

PP100400

NypDII

Aminotransferase

NppDII

96

PP100400

NypDI

Glycosyltransferase

NppDI

92

PP100821

NypE

Thioesterase

NppE

92

PP100306

NypO

Acyl-CoA decarboxylase

NppO

96

PP100306

NypRIV

LuxR transcriptional regulator

NppRIV

93

PP100306

NypM

Hypothetical protein

NppM§

82


*Proposed function of full length nystatin P1 biosynthetic (nyp) genes present in the draft genome of Pseudonocardia sp. P1 (Genbank accession ADUJ00000000).

† NypY is a glycosyltransferase unique to the nystatin P1 biosynthetic gene cluster and is not orthologous to proteins in the nystatin-like Pseudonocardia polyene (NPP) biosynthetic gene cluster from P. autotrophica (AC = EU108007) or the nystatin biosynthetic gene cluster from Streptomyces nouresi (AC = AF263912).

‡ The nystatin P1 and NPP biosynthetic gene clusters contain a putative metallophosphoesterase downstream of nypH and nppH, respectively that is not present in the nystatin biosynthetic gene cluster from S. nouresi. This open reading frame was not originally annotated by Kim et al. [15] and we have therefore given the Pseudonocardia P1 ortholog the name of nypZ.

§ nypM encodes a hypothetical protein with high homology to NppM, which is annotated as a putative ferredoxin [15], however amino acid homology-based database searches failed to reveal homology to ferredoxin proteins.

Barke et al. BMC Biology 2010 8:109   doi:10.1186/1741-7007-8-109

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