Additional file 3.

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification of candicidin in S4 extracts. Analysis of S4-derived extracts. Left panel (A), ultraviolet spectrum extracted at RT 8.3 min (see panel B) from the S4 extract. The absorption maxima match those previously reported for candicidin D [12]. Right panel (B), LC-MS analysis of S4 extract. Ion chromatograms extracted for the molecular ion of candicidin D (m/z 1109.6) are shown. (C), MS2 analysis of the extracted ion m/z 1109.6. The fragmentation pattern of the antifungal compound from Streptomyces S4 perfectly matched the fragmentation of candicidin as reported previously [12]. The ions highlighted in the Haeder et al. study [12] are labelled in a larger font.

Format: PDF Size: 92KB Download file

This file can be viewed with: Adobe Acrobat Reader

Barke et al. BMC Biology 2010 8:109   doi:10.1186/1741-7007-8-109