Figure 5.

Signal transduction cascades regulate the mitochondrial fusion reaction. (a) HeLa cells were either untreated (left panels), treated with 10 μM forskolin for 30 min or with 1.5 μM staurosporin for 4 h were fixed and stained with anti-prdx3 antibodies to label mitochondria. Bottom panels show a 3× magnification of the boxed areas. Scale bars are 10 μ. (b) Renilla luciferase complementation is shown for mitochondrial fusion reactions containing 5 mg/mL sHeLa cytosol, or cytosols treated with forskolin or staurosporin, as indicated. The addition of 10 μM forskolin directly to the fusion reaction does not have any effect. Apyrase and 4°C controls are also shown. (b) As in b, including a control where HeLa cells were incubated with 10 μM forskolin for 30 min in the presence of its inhibitor H89 (10 μM, 1 h pre-incubation) prior to harvesting the cytosol for addition into the cell free assay.

Schauss et al. BMC Biology 2010 8:100   doi:10.1186/1741-7007-8-100
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