Ultrastructural quantification of mitochondrial fusion in vitro. Fusion reactions performed in the absence of cytosol were scaled up to 50 μL and incubated either at 37° or 4°C. Mitochondria were re-isolated and embedded in low-melting agarose for electron microscopy analysis. (a) A representative section from the reaction incubated at 4°C (left panel), or 37°C (right panel). Asterisks point to intact, healthy mitochondria. Scale bars are 500 nm. (b) Quantification of the mitochondrial size, binned into 300 nm units. The data are representative of >300 mitochondria in each condition.
Schauss et al. BMC Biology 2010 8:100 doi:10.1186/1741-7007-8-100