Bone marrow transplantation rescues THOC5/FMIP depleted mice from death. (A) Bone marrow (BM) transplantation: 106 bone marrow cells obtained from THOC5/FMIP (flox/flox) mice (n = 14) were transferred into six-to-eight-week-old nonablated Mx-cre THOC5/FMIP (flox/flox) mouse for each and one day after transplantation recipient mice were injected with 250 μg of poly (I:C). Injection was performed i.p. three times at two-to-three-day intervals. No transplantation: Mx-cre THOC5/FMIP (flox/flox) mice without BM transplantation were injected with poly (I:C). (B) Bone marrow cells were spun down onto glass slides and then stained with May-Grunwald Giemsa and hematoxylin. Bone marrow cells were derived from 1) non-treated Mx-cre THOC5/FMIP (flox/flox) mouse. 2) a poly (I:C) injected normal bone marrow cell recipient Mx-cre THOC5/FMIP (flox/flox) mouse which died 18 days after the first injection (Nonsurvivor). 3) a poly (I:C) injected normal bone marrow cell recipient Mx-cre THOC5/FMIP (flox/flox) survivor (eight weeks after the first injection) (Survivor).
Mancini et al. BMC Biology 2010 8:1 doi:10.1186/1741-7007-8-1